M3913
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M3913
Sigma
SigmaMarker™
Low Range, mol wt 6,500-66,000 Da
| MDL number: | MFCD00165844 |
Description
| Application | SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining. |
| Other Notes | Protein (mol. wt.) Aprotinin, bovine lung (6,500) α-Lactalbumin, bovine milk (14,200) Trypsin inhibitor, soybean (20,000) Trypsinogen, bovine pancreas (24,000) Carbonic anhydrase, bovine erythrocytes (29,000) Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000) Ovalbumin, chicken egg (45,000) Albumin, bovine serum (66,000) |
| Will yield eight protein bands with Coomassie Blue staining: aprotinin, α-lactalbumin, trypsin inhibitor, trypsinogen, carbonic anhydrase, glyceraldehyde-3-phosphate dehydrogenase, ovalbumin and albumin | |
| Reconstitution | Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 0.5% dithiothreitol, 2% SDS, and 0.0005% bromophenol blue. |
| Legal Information | SigmaMarker is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| form | lyophilized powder |
| usage | sufficient for 10-20 large gels (16 × 18 cm) |
| sufficient for 20-30 mini-gels (10 × 10 cm) | |
| vial sufficient for 50-100 PhastGels | |
| mol wt | mol wt 6,500-66,000 Da |
| storage temp. | 2-8°C |
Safety
References
| reference | Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, (1970) Abstract |
| Schagger, H. and von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379, (1987) |
